Student Profile

Contact Email: t.chippendale@keele.ac.uk

Undergraduate Degree: MEng Biochemical Engineering (UCL)

PhD Summary

The research project was initiated with the goal of developing a new application for the established trace gas analysis technique, selected ion flow tube mass spectrometry (SIFT-MS), viz. monitoring the headspace of large scale human cell cultures in real time; particularly those of importance to the field of regenerative medicine.
Some of the major activities that were undertaken as part of the project include analysis of the gas phase above various cultures of primary and immortalised human cell types using SIFT-MS,
culture of human mesenchymal stem cells and hepG2 hepatocarcinoma cells on microcarriers in spinner flasks bioreactors and a larger (1L) disposable bag-type bioreactor and the development of headspace/gas phase sampling methodologies for the analysis of emitted compounds.
A study of ethanol/acetaldehyde and DMSO metabolism by cultured human cell types. Aldehyde dehydrogenase inhibitors (dissolved in DMSO) were applied to the cells and the changes in acetaldehyde and dimethyl sulphide concentrations were detected via SIFT-MS analysis of the culture headspace.
Analysis of the gas/vapour compounds emitted by microbial cell cultures. Of particular interest is a study of the volatile compounds emitted into the gas phase by E. coli (JM109), when cultured in two different media. In this study, the real time capability of SIFT-MS was also utilised to monitor the production of these compounds in the gas phase above one such culture.

 Publications, Presentations and Awards

Publications

• Smith D, Chippendale TWE, Španěl P. Selected ion flow tube, SIFT, studies of the reactions of H3O+, NO+ and O2+ with some biologically active isobaric compounds in preparation for SIFT-MS analyses. Int. J. Mass Spectrom 303 (2011) pp.81–89.
• Španěl P, Dryahina K, Rejšková A, Chippendale TWE, Smith D. Breath acetone concentration; biological variability and the influence of diet. Physiol. Meas. 32 (2011) pp.N23–N31.
• Španěl P, Shestivska V, Chippendale TWE, Smith D. Determination of the Deuterium Abundances in Water from 156 to 10,000 ppm by SIFT-MS. J. Am. Soc. Mass. Spectrom. 22 (2011) pp.179-186.
• Chippendale TWE, Španěl P, Smith D. Time-resolved selected ion flow tube mass spectrometric quantification of the volatile compounds generated by E. coli JM109 cultured in two different media. Rapid Commun. Mass Spectrom. 25 (2011) pp.2163–2172.
• Chippendale TWE, Hu B, El Haj AJ, Smith D. A study of enzymatic activity in cell cultures via the analysis of volatile biomarkers. Analyst 137 (2012) pp.4677-4685.
• Rutter A, Chippendale TWE, Španěl P, Smith D, Sulé-Suso J. Quantification by SIFT-MS of acetaldehyde released by lung cells in 3D models. Analyst 138 (2013) pp.91-95.
• Smith D, Chippendale TWE, Španěl P. Minimising the effects of isobaric product ions in SIFT-MS quantification of acetaldehyde, dimethyl sulphide and carbon dioxide. Curr. Anal. Chem. (in press).
• Smith D, Chippendale TWE, Dryahina K, Španěl P. Nose-exhaled breath analysis by SIFT-MS; mouth contamination and the influence of exercise. Curr. Anal. Chem. (in press).
• Chippendale TWE, Coopman K, Rafiq Q, Hewitt CJ, El Haj AJ. Isolation of mesenchymal stem cells from bone marrow aspirate. In: Comprehensive Biotechnology, 2nd Edition, Vol 5 (2011), pp115-123 ‘Medical Biotechnology and Healthcare’ Ed: Moo-Young M.

Presentations

• Chippendale TWE, Smith D, Coopman K, Hewitt CJ, El Haj AJ. Use of SIFT-MS for the on-line monitoring of stem cell production in stirred-tank bioreactors. ESACT-UK Meeting (2010), 含羞草视频.
• Chippendale TWE, Smith D, Španěl P, Coopman K, Hewitt CJ, El Haj AJ. Selected ion flow tube mass spectrometry as a tool for monitoring volatile compounds in the headspace above mesenchymal cell cultures. TERMIS EU Annual Meeting. Granada, Spain. 7-10 June 2011.
• Chippendale TWE, Smith D, Španěl P, Coopman K, Hewitt CJ, El Haj AJ. Selected ion flow tube mass spectrometry as a tool for monitoring volatile compounds in the headspace above mesenchymal cell cultures. CDT-Joint Conference, Leeds University, July 2011.

Current Employment

Currently conducting research at Keele University along two strands; one relating to the development of a cell counting method based on gas phase analysis of human cell cultures and the other on analysing the volatile compounds emitted into the headspace of cultured microbial cells that commonly infect the lungs of cystic fibrosis sufferers, as a part of a larger project aimed at using breath analysis to diagnose the presence of specific respiratory tract infections

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